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About This Item
form
resin
crosslinking
cross-linked
packaging
pack of 5 mL
manufacturer/tradename
Cytiva 17-0618-01
storage condition
(20% Ethanol)
parameter
<0.1 Mpa pressure, 150-250 cm/hr flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
4% cross-linked agarose
matrix active group
Recombinant streptococcal protein G lacking the albumin-binding region, produced in E. coli
average diameter
90 μm
cleaning in place
2-10
working range
3-9
capacity
≥20 mg binding capacity(human IgG/mL resin)
separation technique
affinity
storage temp.
2-8°C
General description
Protein G Sepharose™ 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose™ 4 Fast Flow. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose™ 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.
As member of the BioProcess media range, Protein G Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Binding specificities that complement Protein A media.
- Binds a broad range of IgG species and subclasses.
- Multi-point attachment minimizes ligand leakage.
- Used in a range of research applications.
Preparation Note
Analysis Note
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Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Protocols
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
Articles
实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
本页介绍了高效的色谱柱填充和抗体亲和层析的制备。
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GE17-0618-01 | 04061837620522 |