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H3784

HEPES sodium salt

Name: HEPES sodium salt
Brand: SIGMA

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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About This Item

Empirical Formula (Hill Notation):

C₈H₁₇N₂NaO₄S

CAS Number:

75277-39-3

Molecular Weight:

260.29

NACRES:

NA.25

PubChem Substance ID:

24895595

UNSPSC Code:

12161700

EC Number:

278-169-7

MDL number:

MFCD00036463

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Properties

grade

BioPerformance Certified

Quality Level

400

assay

≥99.0%

form

powder

storage condition

dry at room temperature

technique(s)

activity assay: suitable, cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

color

white

pH

10-12 (1 M in water)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 0.1 M, clear, colorless

density

1.19 g/cm³ at 20—25 °C, 1.21 g/cm³ at 20—25 °C

absorption

≤0.02 at 280 in H₂O at 0.1 M, ≤0.04 at 260 in H₂O at 0.1 M

suitability

suitable for component for culture media, suitable for molecular biology

application(s)

cell analysis
diagnostic assay manufacturing
general analytical
life science and biopharma

foreign activity

DNase, RNase, NICKase, protease, none detected

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

Description

General description

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer with a remarkable pH buffering capacity between 6.8 and 8.2 (pKa of 7.48 at 25°C). Widely recognized for its versatility, HEPES is extensively employed in cell biology, biochemical, and biological research. Notably, its neutrality towards metal ions makes it a preferred choice for solutions with metal ions, acting as a "non-coordinating buffer."

In cell culture, HEPES excels in maintaining stable physiological pH even amidst fluctuating carbon dioxide concentrations, outperforming bicarbonate buffers (NaHCO₃). Moreover, HEPES showcases valuable properties in various biological and biochemical processes, such as isoelectric focusing for protein separation and minimal interference in DNA-restriction enzyme reactions, making it indispensable across diverse research applications, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, exceptional buffering capacity, and minimal interaction with molecules establish HEPES as a key tool in various research domains.

Application

HEPES sodium salt has been used:

to supplement the HSOF medium
as a buffer component of HCZB medium in egg and embryo culture
as a buffer component in cell culture

Features and Benefits

High-purity product for biochemical and biological research
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
Suitable for Cell culture
Tested for Endotoxins and Total Aerobic Microbial Count
Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease

Physical form

Lyophilized, spray dried, or crystallized material.

Other Notes

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c