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H4034

HEPES

Name: HEPES
Brand: SIGMA

BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

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Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

Empirical Formula (Hill Notation):

C₈H₁₈N₂O₄S

CAS Number:

7365-45-9

Molecular Weight:

238.30

Beilstein:

883043

EC Number:

230-907-9

MDL number:

MFCD00006158

PubChem Substance ID:

24895616

NACRES:

NA.25

grade

BioPerformance Certified

Quality Level

400

Assay

≥99.5% (titration)

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

useful pH range

6.8-8.2

pKa (25 °C)

7.5

cation traces

Fe: ≤5 ppm

absorption

≤0.05 at 260 in H₂O at 0.1 M
≤0.05 at 280 in H₂O at 0.1 M

application(s)

diagnostic assay manufacturing

foreign activity

DNase, RNase, NICKase, protease, none detected

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Biological Buffers

General description

HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:

To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines
As a component of platelet suspension buffer
To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
For the adjustment and maintenance of pH of biological solutions
As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
As a component of keratinocyte and fibroblast culture medium

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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