Monoclonal Anti-HIF-1α antibody produced in mouse

Hypoxia-inducible factor-1 (HIF-1) is a 120kD basic helix-loop-helix protein that belongs to the PAS superfamily of transcription factors. It has a crucial role in activating transcription of hypoxia-inducible genes. It can also activate the expression of the gene coding for NIP3; which subsequently induces cell apoptosis under persistent oxygen deprivation. Monoclonal anti-HIF-1α antibody can be used in immunostaining to detect HIF-1α localization. It can also be used in western blotting. Mouse anti-HIF-1α antibody reacts specifically with HIF1 α subunit of sheep, mouse, rat, ferret and human.

peptide corresponding to amino acids 432-528 of the human HIF-1α protein.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal anti-HIF-1α antibody (diluted 1: 500) can be used in immunofluorescence confocal microscopy. It can also be used in in immunoblotting, immunoprecipitation and immunohistochemistry.

Solution in 0.02 M phosphate buffer containing 0.25 M sodium chloride and 0.1% sodium azide.

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