H9269
Hanks′ Balanced Salt solution
With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
HBSS
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: 0.35 g/L
phenol red: 0.011 g/L
glucose: 1.0 g/L (Dextro)
shipped in
ambient
storage temp.
room temp
General description
Formulation: click here.
Application
Hanks′ balanced salt solution has been used:
- in starvation experiments for mammalian cells
- to collect and maintain brain pieces for primary glial cell culture
- to wash femoral tissue and bone marrow (BM) to culture mesenchymal stem cells
Biochem/physiol Actions
Balanced salt solution (BSS) in cell culture is multifaceted and has various functions. It serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. BSS provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It combines with carbohydrates, such as glucose to act as the principal energy source for cell metabolism. BSS also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
该96孔3D球体BME细胞侵袭检测试剂盒利用符合3D培养要求的96孔球状体形成平板以及专门的球状体形成ECM来促进细胞的聚集和球状体形成。
3D Spheroid BME Cell Invasion Assay utilizes specialized plates and ECM for cell aggregation and spheroid formation in vitro.