H9394
Hanks′ Balanced Salt solution
Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
HBSS
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L
shipped in
ambient
storage temp.
room temp
General description
Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem.
Application
Hanks′ Balanced Salt solution has been used:
- as a component of the culture medium for culturing of mouse cortical slices
- as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs)
- for in situ perfusions of mice liver
Biochem/physiol Actions
Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
胰蛋白酶可用于从培养表面去除贴壁细胞。从培养基底物除去细胞的最常用方法是用胰蛋白酶或胰蛋白酶/EDTA溶液处理。
StableCell™胰蛋白酶溶液设计用于执行标准胰蛋白酶溶液所实现的细胞解离，但不同的是前者无需等分、冷冻和解冻。这样可以节省大量时间。
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.