HSRT100
Enhanced Avian HS RT-PCR Kit
Flexible kit for one-step or two-step RT-PCR
Quality Level
usage
sufficient for 100 reactions
feature
dNTPs included
hotstart
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
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Related Categories
General description
One-step: In a single tube, eAMV™ RT and AccuTaq LA act sequentially to first produce cDNA and then immediately amplify by PCR. This provides quick, sensitive analysis of RNA.
Two-step: Each reaction is individually optimized for greater yields with high fidelity, when protocol requires multiple amplifications, or if maximum yield is more important than maximum convenience.
Application
Features and Benefits
- Greater transcription lengths than other reverse transcriptases, generating cDNA up to 14.1 Kb.
- Higher sensitivity for detecting low abundance messages. eAMV™ RT is able to transcribe RNA that other reverse transcriptases cannot detect.
- Unsurpassed transcription through difficult secondary structure.
- Increased sensitivity, specificity and yield from JumpStart AccuTaq LA DNA Polymerase.
Other Notes
Legal Information
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
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Articles
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.
Protocols
The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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