I6529
IMDM
With sodium bicarbonate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM
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About This Item
Product Name
Iscove′s Modified Dulbecco′s Medium, With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Iscove′s Modified Dulbecco′s Medium contains selenium, additional amino acids and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate, and is a modification of Dulbecco′s Modified Eagle′s medium (DME). Iscove′s medium can be used to support murine B lymphocytes, bone marrow hemopoietic tissue, B cells stimulated with lipopolysaccharide, T lymphocytes, and various hybrid cells.
Application
Iscove′s Modified Dulbecco′s Medium has been used to culture:
- human hepatocellular carcinoma (HCC) cell line HepG2 as monolayers
- to culture synovial tissue samples
- bacterial strain inoculum for stimulation of immune cell experiments
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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