ISO2
Mouse Monoclonal Antibody Isotyping Reagents
sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)
Synonym(s):
Mouse Antibody Isotyping Kit
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About This Item
Storage temp.:
2-8°C
Shipped in:
wet ice
Quality Level
usage
sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)
shipped in
wet ice
storage temp.
2-8°C
General description
The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.
Application
Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA). It may be used in Ouchterlony immunodiffusion assays.
Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.
Biochem/physiol Actions
ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.
Features and Benefits
- May be used in a variety of assay formats
- Suitable for all antibody forms
- Determines all mouse IgG subclasses, IgA, and IgM
Kit Components Only
Product No.
Description
- goat antisera to mouse IgA .2 mL
- goat antisera to mouse IgG3 .2 mL
- goat antisera to mouse IgG1 .2 mL
- goat antisera to mouse IgG2a .2 mL
- goat antisera to mouse IgG2b .2 mL
- goat antisera to mouse IgM .2 mL
Storage Class
10 - Combustible liquids
Regulatory Information
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Protocols
ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| ISO2-1KT | 04061833866184 |