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Merck
CN

L0288

Lipid Mixture 1, Chemically Defined

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

lipid mixture

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352211
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sterility

sterile-filtered

Quality Level

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

storage temp.

2-8°C

General description

Lipid mixture 1, Chemically defined is recommended for use in cell culture at 1 to 10 mL per liter of medium.

Application

Lipid Mixture 1, Chemically Defined has been used:

  • as a component of bovine serum albumin (BSA) containing Bracket-Oliphant (BO) medium for the resuspension of sperm pellet
  • as a supplement in spheroid media for culturing primary human subcutaneous stromal vascular fraction cells
  • to prepare a cell steatosis model for culturing of mouse and human hepatocytes

Other Notes

Contains non-animal derived fatty acids (2 μg/ml arachidonic and 10 μg/ml each linoleic, linolenic, myristic, oleic, palmitic and stearic), 0.22 mg/ml cholesterol from New Zealand sheep′s wool, 2.2 mg/ml Tween-80, 70 μg/ml tocopherol acetate and 100 mg/ml Pluronic F-68 solubilized in cell culture water.
The final product contains 10% ethanol by volume.


Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Articles

不饱和脂肪酸、油酸和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统(用于生物制造包括单克隆抗体在内的异源蛋白)的性能。

棕榈酸等脂肪酸和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统(用于生物制造包括单克隆抗体在内的异源蛋白)的性能。

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

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Nature, 531(7592), 53-58 (2016-03-05)
Little is known about how pro-obesity diets regulate tissue stem and progenitor cell function. Here we show that high-fat diet (HFD)-induced obesity augments the numbers and function of Lgr5(+) intestinal stem cells of the mammalian intestine. Mechanistically, a HFD induces



Global Trade Item Number

SKUGTIN
L0288-100ML04061833945650