M0644
Minimum Essential Medium Eagle
Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
MEM
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes
shipped in
ambient
storage temp.
2-8°C
General description
Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
Application
Minimum essential medium Eagle has been used to:
- maintain natural killer (NK) cell lines
- culture Leishmania donovani strains
- culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles
Preparation Note
Formulated to contain 10.1 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Articles
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.