M0650
Medium 199
With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X
Synonym(s):
Cell growth medium, M199 Medium, TCM 199
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
Medium 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- to culture human mesothelial cells
- as a component of the holding medium for the collection of embryos from pluriparous Boer goat
- in the preparation of spheroid-embedded collagen matrices
Preparation Note
Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.