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M2520

Medium 199

HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

M199 Medium, TCM 199

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
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Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

shipped in

ambient

storage temp.

2-8°C

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used to:

  • maintain the cumulus-oocyte complex (COC) before maturation and for culturing of these oocytes for in vitro maturation
  • to rinse the aspiration line during the collection of the ovum from German Holstein cows
  • to culture cardiac endothelial cells from rats

Preparation Note

Formulated to contain 14.7 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG7295
SLCB6503
SLBW5714
SLBR1506V
SLBH4835V

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Protocols

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

钙在细胞培养中的应用

钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Jarosław Wieczorek et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-16)
The laparoscopic method of recovering oocytes in goats and sheep is one of the minimally invasive methods used in the biotechnology of animal reproduction. It allows for good quality oocytes that are suitable for in vitro maturation and fertilization to
Mao Ye et al.
Scientific reports, 4, 4681-4681 (2014-04-16)
The highly specialized endothelial cells in brain capillaries are a key component of the blood-brain barrier, forming a network of tight junctions that almost completely block paracellular transport. In contrast to vascular endothelial cells in other organs, we show that
Sait Sendag et al.
Animal reproduction science, 106(1-2), 208-214 (2008-02-26)
The goal of the present study was to compare the ovarian response, oocyte yields per animal, and the morphological quality of oocytes collected by ultrasound guided follicular aspiration from Holstein cows treated either with FSH or eCG. Twenty four normal
View All Related Papers

Global Trade Item Number

SKUGTIN
M2520-10X1L04061837847325