M3769
Medium 199
Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture
Synonym(s):
M199 Medium, TCM 199
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About This Item
NACRES:
NA.71
UNSPSC Code:
12352207
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
- to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
- to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)
Preparation Note
Formulated to contain 9.4 grams of powder per liter of medium.
Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Articles
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Agnieszka Blitek et al.
Theriogenology, 127, 88-101 (2019-01-25)
Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor family of ligand-dependent transcription factors. PPARs are important regulators of glucose and fatty acid metabolism, apoptosis, angiogenesis, cell proliferation and differentiation, and immune response. Their possible role in the female
Vania Cristina Santos et al.
PloS one, 9(11), e111241-e111241 (2014-11-05)
Some reports have described the interference of Leishmania on sand flies physiology, and such behavior most likely evolved to favor the development and transmission of the parasite. Most of these studies showed that Leishmania could modulate the level of proteases
Diane G Edmondson et al.
mBio, 12(1) (2021-02-25)
The bacterium that causes syphilis, Treponema pallidum subsp. pallidum, has now been cultured in vitro continuously for periods exceeding 3 years using a system consisting of coculture with Sf1Ep rabbit epithelial cells in TpCM-2 medium and a low-oxygen environment. In
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M3769-50L | 04061834050568 |
| M3769-10X1L | 04061834050551 |