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About This Item
Empirical Formula (Hill Notation):
C22H28O13
CAS Number:
Molecular Weight:
500.45
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
276-744-7
MDL number:
Product Name
4-Methylumbelliferyl β-D-cellobioside, glucanase substrate
Quality Level
assay
≥98.00% (TLC)
form
powder
solubility
water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow
storage temp.
2-8°C
SMILES string
CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12
InChI
1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15-,16+,17-,18-,19-,20-,21-,22+/m1/s1
InChI key
PRTGXBPFDYMIJH-MKQZUAMYSA-N
Application
4-Methylumbelliferyl β-D-cellobioside has been used as a substrate:
- to assay cellulase from A. niger
- to determine the extracellular enzyme activity for 1,4-β-cellobiohydrolase
- to assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reesei class II hydrophobins (HFBI)
Biochem/physiol Actions
4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
涉药品监管产品
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