M7633
4-Methylumbelliferyl α-D-galactopyranoside
≥98% (TLC)
Synonym(s):
MU-alpha-GAL
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About This Item
Empirical Formula (Hill Notation):
C16H18O8
CAS Number:
Molecular Weight:
338.31
UNSPSC Code:
12352204
NACRES:
NA.32
PubChem Substance ID:
EC Number:
254-031-1
Beilstein/REAXYS Number:
94674
MDL number:
Quality Level
assay
≥98% (TLC)
form
powder
solubility
water: 50 mg/mL, clear, colorless to very faintly yellow
fluorescence
λex 317 nm; λem 374 nm (pH 9.1), λex 360 nm; λem 449 nm (Reaction product)
storage temp.
−20°C
SMILES string
CC1=CC(=O)Oc2cc(O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)ccc12
InChI
1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m1/s1
InChI key
YUDPTGPSBJVHCN-CHUNWDLHSA-N
Application
4-Methylumbelliferyl α-D-galactopyranoside has been used as an artificial substrate to assay α-galactosidase-A activity.
Biochem/physiol Actions
4-Methylumbelliferyl α-D-galactopyranoside is a fluorogenic substrate for α-galactosidase-A. The enzyme converts the substrate to form a blue fluorescent product, methylumbelliferyl. The fluorescence is measured spectrophotometrically.
Preparation Note
4-Methylumbelliferyl α-D-galactopyranoside is soluble in water (50 mg/ml), yielding a clear, colorless to faint yellow solution with the application of heat.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
涉药品监管产品
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Related Content
S J Park et al.
Applied and environmental microbiology, 61(5), 2027-2029 (1995-05-01)
With a spectrofluorometer, the length of the incubation time required in the fluorogenic assay was reduced to 12 h. The threshold emissions for reading the fluorogenic reaction by the spectrofluorometer were 5 and 10 U for lauryl tryptose broth media
S Raghavan et al.
Journal of inherited metabolic disease, 28(6), 1005-1009 (2006-01-26)
Globoid cell leukodystrophy is an inherited metabolic disorder of the central nervous system caused by deficiency of the lysosomal enzyme galactocerebrosidase. Haematopoietic stem cell transplantation is the only available effective treatment. The engraftment from normal donors provides competent cells able
Y Oda et al.
Analytical biochemistry, 254(1), 41-48 (1998-01-31)
A fluorometric assay of lectin binding to yeast cells is reported. The relative amount of biotinylated lectins bound to the yeast cells was estimated by enzyme activity using 4-methylumbelliferyl-beta-D-galactoside as a substrate for the lectin-bound beta-galactosidase through biotin-avidin interaction. Binding
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M7633-25MG | 04061835557745 |
| M7633-100MG | 04061835546176 |
| M7633-250MG | 04061835557738 |