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M7653

Medium 199

Name: Medium 199
Brand: SIGMA

With Hanks′ salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

Medium 199, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO₃: yes
Hanks’ salts (2% CO₂): yes
sodium pyruvate: no
phenol red: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Modification of the original Medium 199.Earle′s salts are replaced with Hanks′ salts. Hanks′ salts use a lower concentration of bicarbonate and different concentrations and salt forms of the essential inorganic ions.

Preparation Note

Supplement with 0.1 g/L L-glutamine.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Articles

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.