M9004
Malic Dehydrogenase from bovine heart
ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)
Synonym(s):
L-Malate:NAD+ oxidoreductase, MDH
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-622-5
MDL number:
Specific activity:
2000-4000 units/mg protein (modified Warburg-Christian)
Biological source:
bovine heart
biological source
bovine heart
Quality Level
form
ammonium sulfate suspension
specific activity
2000-4000 units/mg protein (modified Warburg-Christian)
mol wt
35 kDa
impurities
≤0.01% Glutamic-Oxalacetic Transaminase, ≤0.01% Glutamic-Pyruvic Transaminase
storage temp.
2-8°C
General description
Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.
Application
Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.
Physical form
Suspension in 3 M (NH4)2SO4 - 0.01 M KH2PO4 solution, pH 7.3
Other Notes
One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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Protocols
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
Articles
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Related Content
Enika Nagababu et al.
Methods in molecular biology (Clifton, N.J.), 610, 165-180 (2009-12-17)
Reactive oxygen species are implicated in many human diseases and aging process. Much of the evidence is based on experimental data indicating increasing rates of lipid peroxidation in disease states and the ameliorating effects of antioxidants. It is becoming increasingly
E M Gregory
The Journal of biological chemistry, 250(14), 5470-5474 (1975-07-25)
Bovine mitochondrial malate dehydrogenase (EC 1.1.1.37) was inactivated by the specific modifications of a single histidine residue upon reaction with iodoacetamide. NADH protected against this loss of activity and reaction with the histidine residue, suggesting that the histidine is at
Flow injection system for on-line monitoring of fumaric acid in biological processes
Rhee, J.
Analytica Chimica Acta, 499, 71-80 (2003)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M9004-5KU | 04061834063797 |
| M9004-10KU | 04061834063780 |
| M9004-25KU | 04061832731001 |