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MOD50

Sigma-Aldrich

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

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Synonym(s):
Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification
EC Number:
231-548-0
NACRES:
NA.54

usage

sufficient for 50 reactions

storage temp.

20-25°C

Related Categories

General description

The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.

Application

Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.

Features and Benefits

  • Only 50 picograms of DNA or 20 cells are required
  • Procedure takes less than 2 hours
  • Greater than 99% conversion rate
  • Extremely low degradation
  • Option of convenient one-step protocol
  • Consistent and reproducible Bisulfite Modification
  • Can be used with genomic, endonuclease digested, and FFPE DNA

Storage and Stability

All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, naturalSDS

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H290 - H302 - H314 - H412

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Supplementary Hazards

EUH031

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

危险化学品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

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In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?

    This kit can be used for 50 reactions.

  4. What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?

    DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.

  5. Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?

    Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.

  6. Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?

    The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.

  7. Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?

    Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Susanne Edelmann et al.
Methods in molecular biology (Clifton, N.J.), 1675, 45-60 (2017-10-21)
Bisulfite sequencing (BS-seq) enables the detection of DNA methylation at cytosine residues (5mC) at single-nucleotide resolution. For many applications, a limiting factor of conventional BS-seq protocols is the high amount of DNA required, since the treatment with bisulfite causes severe
M Y Follo et al.
Leukemia, 26(5), 943-950 (2011-10-29)
Phosphoinositide-phospholipase C (PI-PLC) beta1 can be considered a specific target for demethylating therapy in high-risk myelodysplastic syndrome (MDS) patients, as azacitidine treatment has been associated with a PI-PLCbeta1-specific promoter demethylation, and induction of PI-PLCbeta1 gene and protein expression. However, little
Javed Hussain Choudhury et al.
Methods in molecular biology (Clifton, N.J.), 1726, 111-122 (2018-02-23)
DNA methylation plays a decisive role in the regulation and control of gene expression. DNA methylation is a covalent modification, in which a methyl group is attached to the 5th carbon of the cytosine ring of a CpG dinucleotide that
MicroRNA-20 induces methylation of hepatitis B virus covalently closed circular DNA in human hepatoma cells
In Young Moon, et al.
Molecular Medicine Reports, 2285-2293 (2019)
Histone H3 Lysine 36 Trimethylation Is Established over the Xist Promoter by Antisense Tsix Transcription and Contributes to Repressing Xist Expression
Ohhata T, et al.
Molecular and Cellular Biology null
View All Related Papers

Articles

DNA Methylation and Bisulfite Conversion

There are several common ways to determine whether a gene contains methylated DNA. Since mammalian methylation occurs at cytosines, researchers take advantage of the fact that methylated cytosine (meC) is stable to bisulfite treatment but unmethylated cytosine is transformed to uracil under the same conditions.

Related Content

Imprint ® DNA Modification Kit (MOD50) Protocol

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service