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P0913

Polyinosinic–polycytidylic acid sodium salt

γ-irradiated, 10% Poly (I:C) with buffer salts, TLR ligand tested

Synonym(s):

Poly (I:C), Poly(I) • Poly(C)

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About This Item

CAS Number:
42424-50-0
UNSPSC Code:
41106305
NACRES:
NA.51
MDL number:
MFCD00131984
Assay:
(Free nucleotides NMT 1% (TLC)), ≥99% (less than 1% free nucleotides, TLC)
Biological source:
synthetic (organic)
Form:
lyophilized powder
Solubility:
H2O: 10 mg/mL
Storage temp.:
−20°C
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biological source

synthetic (organic)

Quality Level

300

sterility

γ-irradiated

assay

(Free nucleotides NMT 1% (TLC)), ≥99% (less than 1% free nucleotides, TLC)

form

lyophilized powder

storage condition

desiccated

solubility

H2O: 10 mg/mL

storage temp.

−20°C

SMILES string

[Na].[P](=O)(OC[C@H]3O[C@H]([C@@H]([C@@H]3O)O)[n]4c5ncnc(c5nc4)O)(O)O.[P](=O)(OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)N2C=CC(=NC2=O)N)(O)O

InChI

1S/C10H13N4O8P.C9H14N3O8P.Na/c15-6-4(1-21-23(18,19)20)22-10(7(6)16)14-3-13-5-8(14)11-2-12-9(5)17;10-5-1-2-12(9(15)11-5)8-7(14)6(13)4(20-8)3-19-21(16,17)18;/h2-4,6-7,10,15-16H,1H2,(H,11,12,17)(H2,18,19,20);1-2,4,6-8,13-14H,3H2,(H2,10,11,15)(H2,16,17,18);/t

InChI key

GETQKWBYQAUFQM-VRQAYDGLSA-N

Application

TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.

Biochem/physiol Actions

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.

Preparation Note

The product requires ionic strength to maintain the double-strand structure. To prevent denaturation, reconstitute in solutions with physiological salt concentrations (e.g., saline solution). Reconstitution may require heating (50 °C) and cooling to achieve re-annealing.

Other Notes

Double-stranded homopolymer.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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