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P246

Sigma-Aldrich

Monoclonal Anti-PSD95 antibody produced in mouse

clone 7E3-1B8, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Anti-MRD62, Anti-PSD95, Anti-SAP-90, Anti-SAP90
MDL number:
MFCD01324382

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7E3-1B8, monoclonal

form

buffered aqueous solution

mol wt

antigen 95 kDa

species reactivity

rat

technique(s)

immunofluorescence: 1:500
immunoprecipitation (IP): suitable
western blot: 1:2,000

isotype

IgG2a

UniProt accession no.

P31016

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Dlg4(29495)

General description

DLG4 (discs large MAGUK scaffold protein 4) gene codes for the postsynaptic density-95 (PSD95) protein. Postsynaptic density 95 (PSD-95), a synaptic scaffolding protein is also a membrane-associated guanylate kinase (MAGUK) family protein. It has three PDZ domains on the N terminus followed by a Src homology 3 (SH3) domain and a guanylate kinase (GK) domain. This gene is located on human chromosome 17p13.1.

Specificity

Reacts with both the recombinant and native rat PSD95. By immunofluorescence with rat hippocampal cells, a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with its proposed role in receptor clustering is observed. Fixation with cold methanol is recommended.

Immunogen

recombinant rat PSD95 (post synaptic density 95 kDa).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Postsynaptic density 95 (PSD-95) plays a major role in bidirectional synaptic plasticity, an important process, essential for learning and memory. It exhibits increased dynamics upon induction of plasticity.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Capping of the N-terminus of PSD-95 by calmodulin triggers its postsynaptic release
Yonghong Zhang
The Embo Journal (2014)
Human postsynaptic density-95 (PSD95): location of the gene (DLG4) and possible function in nonneural as well as in neural tissues
Stathakis D G, et al.
Genomics, 44(1), 71-82 (1997)
Decreased PSD95 expression in medial prefrontal cortex (mPFC) was associated with cognitive impairment induced by sevoflurane anesthesia* #
Yun-Zhi Ling
Journal of Zhejiang University. Science. B (2015)
Xiao-Mei Liu et al.
The Journal of biological chemistry, 281(25), 17432-17445 (2006-04-21)
Previous studies have suggested that glutamate receptor 6 (GluR6) subunit- and JNK-deficient mice can resist kainate-induced epileptic seizure and neuronal toxicity (Yang, D. D., Kuan, C.-Y., Whitmarsh, A. J., Rinoćn, M., Zheng, T. S., Davis, R. J., Rakic, P., and
Malwina Lisek et al.
Frontiers in cellular neuroscience, 11, 181-181 (2017-07-14)
Ketamine causes psychotic episodes and is often used as pharmacological model of psychotic-like behavior in animals. There is increasing evidence that molecular mechanism of its action is more complicated than just N-methyl-D-aspartic acid (NMDA) receptor antagonism and involves interaction with
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