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P4417

Sigma-Aldrich

Phosphate buffered saline

tablet

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Synonym(s):
PBS
MDL number:
MFCD00131855
eCl@ss:
32129211
NACRES:
NA.25

form

tablet

pH

7.2-7.6 (1 tablet/200 mL)

solubility

water: soluble

application(s)

diagnostic assay manufacturing

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General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Application

Phosphate buffered saline has been used-
  • for the production of platinum nanoparticles (NP-Pt) hydrocolloids
  • for the washing of blood sample and SEM (scanning electron microscope) stubs prior to SEM
  • for preparing the solution of fluorescent lectin wheat germ agglutinin (WGA)
  • for the dilution of capture antibody during ELISA (enzyme linked immunosorbent assay)
  • for suspension of cell pellets during centrifugation
  • in immunoassay procedures

Reconstitution

One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Does Product P4417, Phosphate buffered saline (PBS), contain calcium or magnesium?

    No.  The complete formulation is given in the product listing.  No calcium or magnesium salts are included in the preparation.

  4. Does one tablet of Product P4417, Phosphate buffered saline (PBS), make up 1X or 10X PBS?

    When 1 tablet is reconstituted in 200 ml of water, the resulting solution is 1X PBS.

  5. Can Product P4417, Phosphate buffered saline (PBS), solution be autoclaved?

    Yes.  PBS is not harmed by standard autoclave procedures.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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Structural damage of chicken red blood cells exposed to platinum nanoparticles and cisplatin.
Kutwin M et al
Nanoscale Research Letters, 9(1), 257-257 (2014)
Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins.
Lopez C et al
Food Chemistry, 120.1, 22-33 (2010)
Sputum cathelicidin, urokinase plasminogen activation system components, and cytokines discriminate cystic fibrosis, COPD, and asthma inflammation.
Xiao W et al
Chest, 128(4), 2316-2326 (2005)
M Michaelis et al.
The journal of physical chemistry. B, 123(31), 6694-6704 (2019-07-11)
Although successful in the structural determination of ordered biomolecules, the spectroscopic investigation of oligopeptides in solution is hindered by their complex and rapidly changing conformational ensemble. The measured circular dichroism (CD) spectrum of an oligopeptide is an ensemble average over
Wenwen Yu et al.
Food chemistry, 241, 493-501 (2017-09-30)
The conversion of barley starch to sugars is a complex enzymic process. Most previous work concerned the biotechnical aspect of in situ barley enzymes. However, the interactions among the macromolecular substrates and their effects on enzymic catalysis has been little
View All Related Papers

Protocols

Antibody Purification using Protein A, Protein G, or Protein L Agarose

Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum or ascites the final preparation will contain endogenous host IgG as well as specific antibodies. In general, the presence of this endogenous IgG should not interfere with assays using the antibodies.

Immunofluorescence Labeling of Cells

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

ELISA Procedures

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

Immunoprecipitation Procedure

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

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