Anti-Parkin antibody, Mouse monoclonal

Monoclonal Anti- Parkin (mouse IgG2b isotype) is derived from the hybridoma PRK8 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human Parkin. Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. The parkin gene encodes a protein of 465 amino acid residues with moderate similarity to ubiquitin at the amino-terminus and a ring in between ring fingers (IBR)-ring-finger motif at the carboxyl-terminus. The gene has 12 exons.

Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. It is a RING-type E3 ubiquitin-protein ligase that facilitates the ubiquitination of misfolded proteins and protects from neurotoxic effect caused by these proteins. Monoclonal anti-parkin antibody can be used in microarray and immunoprecipitation assays. It can also be used in western blotting. Mouse anti-parkin antibody reacts specifically with parkin (50kD approx) of rat, hamster, mouse and human.

recombinant human Parkin.

Monoclonal anti-parkin antibody has been used in:

• Enzyme linked immunosorbent assay (ELISA)
• Immunoblotting
• Immunoprecipitation[63}
• Immunocytochemistry
• to label parkin in pull-down assay

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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