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P7998

Alkaline Phosphatase Yellow

alkaline phosphatase substrate, chromogenic liquid

Synonym(s):

Alkaline phosphatase substrate solution (pNPP)

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About This Item

UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.83
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Product Name

Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA, ready to use solution

Quality Level

200

form

liquid

storage temp.

2-8°C

General description

The product is ready to use buffered alkaline phosphatase substrate consist of p-nitrophenylphosphate (pNPP). This is the advanced substrate system contains insoluble stabilizer that enhances the stability and reactivity as well as reduces the base-line absorption. It produces a soluble end product, hence not recommended for blotting or histochemistry applications.

Application

Develops a soluble yellow reaction product that may be read at 405 nm. For endpoint assays, the reaction may be stopped with 3 N NaOH. Recommended for ELISA (microwell) procedures; not recommended for membrane applications.

Physical form

Ready to use

Preparation Note

Contains p-nitrophenyl phosphate, a substrate for alkaline phosphatase.

Disclaimer

 Stable at least one year at 2-8 °C.

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

target_organs

Kidney,Liver,Blood

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Storage Class

12 - Non Combustible Liquids

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL5966
SLCK5052
SLCJ7348
SLCJ6450
SLCJ1764

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Articles

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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Global Trade Item Number

SKUGTIN
P7998-100ML04061838256232