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R8508

Sigma-Aldrich

Ribonucleic acid, transfer from baker's yeast (S. cerevisiae)

buffered aqueous solution

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Synonym(s):
Transfer RNA, tRNA
CAS Number:
9014-25-9
MDL number:
MFCD00132200
NACRES:
NA.55

grade

for molecular biology

Quality Level

200

form

buffered aqueous solution

concentration

9-11 mg/mL

foreign activity

DNase, Nickase, none detected

shipped in

dry ice

storage temp.

−20°C

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General description

Transfer RNA (tRNA) is isolated from baker′s yeast.
Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.

Application

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription. It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation.

Biochem/physiol Actions

Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.

Components

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Jean Lehmann et al.
Nucleic acids research, 41(10), 5494-5502 (2013-04-13)
The structure and function of conserved motifs constituting the apex of Stem I in T-box mRNA leaders are investigated. We point out that this apex shares striking similarities with the L1 stalk (helices 76-78) of the ribosome. A sequence and
Manuel Neeb et al.
Journal of medicinal chemistry, 57(13), 5566-5578 (2014-06-25)
Lead optimization focuses on binding-affinity improvement. If a flat structure-activity relationship is detected, usually optimization strategies are abolished as unattractive. Nonetheless, as affinity is composed of an enthalpic and entropic contribution, factorization of both can unravel the complexity of a
Tanja Kalstrup et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(20), 8272-8277 (2013-05-01)
Atomic-scale models on the gating mechanism of voltage-gated potassium channels (Kv) are based on linear interpolations between static structures of their initial and final state derived from crystallography and molecular dynamics simulations, and, thus, lack dynamic structural information. The lack
Narayana Annaluru et al.
Science (New York, N.Y.), 344(6179), 55-58 (2014-03-29)
Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair
Stefan Klumpp et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(42), 16754-16759 (2013-10-02)
Bacterial growth is crucially dependent on protein synthesis and thus on the cellular abundance of ribosomes and related proteins. Here, we show that the slow diffusion of the bulky tRNA complexes in the crowded cytoplasm imposes a physical limit on
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