S0902
SSC Buffer
for Northern and Southern blotting, powder blend
Synonym(s):
Saline-sodium citrate buffer
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About This Item
NACRES:
NA.25
UNSPSC Code:
41105319
Quality Level
form
powder blend
pH
6.8-7.2 (20-25 °C, 263 mg/L in water)
solubility
water: 263 mg/mL, clear, colorless
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.
Application
Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
- Preparing nucleic acids for hybridization
- Hybridization buffer for Northern and Southern blots
- Washing buffer for Northern and Southern blots
Features and Benefits
- Multi-purpose use in hybridizations
- Easily reconstituted and diluted to any concentration needed
Preparation Note
Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.