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S1629

Sulfatase from Aerobacter aerogenes

Name: Sulfatase from <I>Aerobacter aerogenes</I>
Brand: SIGMA

Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

CAS Number:

9016-17-5

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.1.6.1 (BRENDA, IUBMB)

MDL number:

MFCD00132399

Specific activity:

2-5 units/mg protein (biuret)

Concentration:

10-20 units/mL

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Properties

type

Type VI

Quality Level

200

form

buffered aqueous glycerol solution

specific activity

2-5 units/mg protein (biuret)

mol wt

~41 kDa

concentration

10-20 units/mL

foreign activity

β-Glucuronidase ≤10 U/mL

shipped in

wet ice

storage temp.

−20°C

Description

General description

Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.

Application

Sulfatase from Aerobacter aerogenes has been used:

as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides

Biochem/physiol Actions

Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.

Physical form

Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Global Trade Item Number

SKU	GTIN
S1629-50UN	04061832759685
S1629-10UN	04061836911768