T3069
Trizma® hydrochloride solution
pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture
Synonym(s):
Tris hydrochloride solution
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
Quality Level
grade
BioPerformance Certified, Molecular Biology
form
solution
concentration
2 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)
color
colorless
pH
7.95—8.05, 8.0
useful pH range
7.0-9.0
density
1.07—1.09 g/cm3
absorption
≤0.05 at 290 at 40%
General description
Tris(hydroxymethyl)aminomethane (Tris) has a pH buffering range between 7.0 - 9.0 and is a routine buffer in biological sciences. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. It displays negligible binding towards metal in solutions.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Application
Trizma® hydrochloride solution has been used as a component of lysis buffer for testing on commercially available biological sample preservation paper types. It has also been used as a vehicle and for dissolving leptin in reporter gene assay studies.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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