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T6025

Tris Acetate-EDTA buffer

BioReagent, suitable for electrophoresis

Synonym(s):

TAE buffer

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About This Item

NACRES:
NA.25
PubChem Substance ID:
329826837
UNSPSC Code:
41105319
MDL number:
MFCD00236357
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sterility

0.2 μm filtered

Quality Level

200

product line

BioReagent

form

working solution

technique(s)

electrophoresis: suitable

impurities

DNase, RNase, Protease, none detected

suitability

suitable for electrophoresis, suitable for gel electrophoresis (after dilution to working concentration)

application(s)

diagnostic assay manufacturing

SMILES string

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

HGEVZDLYZYVYHD-UHFFFAOYSA-N

Application

Tris Acetate-EDTA buffer has been used as run buffer for the agarose gel electrophoresis of human papillomavirus DNA and transcribed RNA samples.
TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Solution prepared with 18 megohm water

Other Notes

40 mM Tris acetate, pH approx. 8.3, containing 1 mM EDTA.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCC3584
SLBW5974
SLBQ6542V
SLBM8551V
SLBJ7837V

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An in vitro enzymatic assay to measure transcription inhibition by gallium (III) and H3 5, 10, 15-tris (pentafluorophenyl) corroles
Tang GY, et al.
Journal of Visualized Experiments, 2(97), e52355-e52355 (2015)
Monica Musiani et al.
Nature protocols, 2(10), 2502-2510 (2007-10-20)
PCR is an established technique providing rapid and highly productive amplification of specific DNA sequences. The demand for equally rapid, sensitive and objective methods to achieve detection of PCR products has led to the coupling of PCR with ELISA. PCR-ELISA

Global Trade Item Number

SKUGTIN
T6025-1L04061837348693