T6066
Trizma® base
BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
grade
BioPerformance Certified
Quality Level
Agency
USP/NF
meets EP testing specifications
meets USP testing specifications
description
aminopeptidase substrate
Assay
≥99.9% (titration)
form
crystalline powder
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, protease, none detected
Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested
≤0.2% water (Karl Fischer)
loss
≤0.5% (loss on drying, 110 °C)
pH
10.0-11.5
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
solubility
water: soluble (666 mg/ml, yielding a clear colorless solution)
absorption
≤0.05 at 260 at 1 M
≤0.05 at 290 at 40%
≤0.06 at 280 at 1 M
application(s)
diagnostic assay manufacturing
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
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Related Categories
General description
Application
- As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
- As a component of pre-hybridization buffer in in situ hybridization
- To suspend dried protein before SDS-PAGE protein characterization
- As a component of lysis buffer to wash and lyse cells before immunoprecipitation
- In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
- In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
- In capillary electrochromatography and UV analysis of tocopherols and tocotrienols
Packaging
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.
Other Notes
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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