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T7575

Sigma-Aldrich

Trypsin Singles, Proteomics Grade

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Synonym(s):
Proteomics grade, Trypsin, TPCK treated
NACRES:
NA.56

grade

Proteomics Grade

form

ready-to-use solution

specific activity

≥10,000 BAEE units/mg protein

mol wt

average mol wt 23.29 kDa

optimum pH

~8.0

storage temp.

2-8°C

Related Categories

General description

Experience all of the advantages of Proteomics Grade Trypsin in a convenient, single-use 1 μg package.

Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.

Application

Trypsin Singles, Proteomics Grade have been used for protein digestion to analyse generated peptides via MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry). It has been used for the digestion of proteins to analyse generated peptides via LC-MS (liquid chromatography–mass spectrometry)/MS.

Features and Benefits

  • Convenient, single-use 1 μg size
  • Eliminates repetitive pipetting
  • Reductively methylated to minimize autolytic activity
  • TPCK treated to quench chymotryptic activity
  • Extensively purified

Kit Components Also Available Separately

Product No.
Description
SDS
  • Trypsin Solubilization Reagent

related product

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Regulatory Information

危险化学品
常规特殊物品

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Robert D Gray et al.
American journal of respiratory and critical care medicine, 178(5), 444-452 (2008-06-21)
Markers of inflammatory activity are important for assessment and management of many respiratory diseases. Markers that are currently unrecognized may be more valuable than those presently believed to be useful. To identify potential biomarkers of suppurative and inflammatory lung disease
Daniel W Udwary et al.
Applied and environmental microbiology, 77(11), 3617-3625 (2011-04-19)
Bacteria of the genus Frankia are mycelium-forming actinomycetes that are found as nitrogen-fixing facultative symbionts of actinorhizal plants. Although soil-dwelling actinomycetes are well-known producers of bioactive compounds, the genus Frankia has largely gone uninvestigated for this potential. Bioinformatic analysis of
Michael Costanzo et al.
Science (New York, N.Y.), 327(5964), 425-431 (2010-01-23)
A genome-scale genetic interaction map was constructed by examining 5.4 million gene-gene pairs for synthetic genetic interactions, generating quantitative genetic interaction profiles for approximately 75% of all genes in the budding yeast, Saccharomyces cerevisiae. A network based on genetic interaction
Arun K Ramani et al.
Molecular systems biology, 4, 180-180 (2008-04-17)
The human protein interaction network will offer global insights into the molecular organization of cells and provide a framework for modeling human disease, but the network's large scale demands new approaches. We report a set of 7000 physical associations among
Jeramie Watrous et al.
Molecular bioSystems, 6(2), 376-385 (2010-01-23)
Selective degradation of cellular proteins offers an important mechanism to coordinate cellular processes including cell differentiation, defense, metabolic control, signal transduction and proliferation. While much is known about eukaryotic ubiquitination, we know little about the recently discovered ubiquitin-like protein in
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