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Merck
CN

T9326

Thrombin human

BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE)

Synonym(s):

Factor IIa

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥2,000 units/mg protein
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells
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recombinant

expressed in HEK 293 cells

Quality Level

product line

BioUltra

assay

≥95% (SDS-PAGE)

form

aqueous solution

specific activity

≥2,000 units/mg protein

mol wt

37.4 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... F2(2147)

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess in vitro hemostatic properties of French lyophilized plasma.

Physical form

supplied as a solution in 20 mM MES, pH 6.0, 500 mM choline chloride

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.


Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

This item has



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Articles

凝血酶因子IIa是一种内切丝氨酸蛋白酶,可选择性地裂解纤维蛋白原的Arg -Gly键,形成纤维蛋白并释放纤维蛋白肽A和B。

Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.






Global Trade Item Number

SKUGTIN
T9326-150UN04061837381270