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About This Item
Specific activity:
≥2,000 units/mg protein
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells
General description
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Application
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess in vitro hemostatic properties of French lyophilized plasma.
Physical form
supplied as a solution in 20 mM MES, pH 6.0, 500 mM choline chloride
Other Notes
Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
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Articles
凝血酶因子IIa是一种内切丝氨酸蛋白酶,可选择性地裂解纤维蛋白原的Arg -Gly键,形成纤维蛋白并释放纤维蛋白肽A和B。
Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.
Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T9326-150UN | 04061837381270 |