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About This Item
UNSPSC Code:
12352207
EC Number:
263-718-5
NACRES:
NA.84
form
liquid
Quality Level
usage
1 kit sufficient for 2,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
protein quantification: suitable
application(s)
cell analysis
detection
detection method
colorimetric, fluorometric
storage temp.
2-8°C
General description
In Vitro Toxicology Assay Kit (Resazurin based) is designed for fluorometrically or spectrophotometrically determining cell number as a function of metabolic activity using the dye resazurin. The resazurin method is simple, accurate, and reproducible
Application
In Vitro Toxicology Assay Kit, Resazurin based has been used:
- in cell viability assay of human primary fibroblasts
- to determine the cell proliferation rate in murine pre-osteoblasts
- to determine the metabolic activity of microfluidic and monolayer cultures
Biochem/physiol Actions
Bioreduction of the dye reduces the amount of its oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
基于细胞的细胞增殖(BrdU、MTT、WST1)、细胞活力和细胞毒性实验,可应用于癌症、神经科学和干细胞研究。
用于维持高质量经验证的无污染细胞培养的质量控制指南。免费下载ECACC手册。
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.