Z2126
ZAP-70 Tyrosine Kinase human
≥90% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells (Histidine tagged), buffered aqueous glycerol solution, ≥150 U/mg
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About This Item
Specific activity:
≥150 U/mg
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected insect cells (Histidine tagged)
Quality Level
recombinant
expressed in baculovirus infected insect cells (Histidine tagged)
assay
≥90% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
≥150 U/mg
mol wt
70 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... ZAP70(7535)
Application
Human ZAP-70 Tyrosine Kinase was used for mapping the phosphorylation sites on LAT (linker for activation of T cells) for investigating the mechanism of activation of signalling proteins in T cells.
Biochem/physiol Actions
ZAP-70 Tyrosine Kinase is a protein belongs to Syk family and is essential for T cell activation. It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis. Additionally, it also plays a pivotal role in LFA-1-dependent T Cell Migration.
ZAP-70 is protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells. It is required for T cell activation. It is activated by Lck-mediated phosphorylation of its tyrosine residues.
Physical form
Solution in 25 mM HEPES, pH 7.5, containing 150 mM NaCl, 0.5 mM DTT, and 50% glycerol.
Other Notes
One unit will phosphorylate 1 nmol polyglutamic acid:tyrosine per min at 37 °C at pH 7.0.
Storage Class
10 - Combustible liquids
wgk
WGK 1
Regulatory Information
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Related Content
C M Eischen et al.
Journal of immunology (Baltimore, Md. : 1950), 159(3), 1135-1139 (1997-08-01)
Activation-induced cell death (AICD) is initiated by the TCR-dependent up-regulation of Fas ligand (FasL) mRNA. The subsequently generated soluble or cell-associated FasL gene products bind Fas, leading to apoptosis of the T cells. Although TCR stimulation is essential to initiate
P E Paz et al.
The Biochemical journal, 356(Pt 2), 461-471 (2001-05-23)
T-cell-receptor (TCR)-mediated LAT (linker for activation of T cells) phosphorylation is critical for the membrane recruitment of signalling complexes required for T-cell activation. Although tyrosine phosphorylation of LAT is required for recruitment and activation of signalling proteins, the molecular mechanism
R D Soede et al.
The Journal of cell biology, 142(5), 1371-1379 (1998-09-10)
The ZAP-70 tyrosine kinase is essential for T cell activation by the T cell receptor. We show that ZAP-70 is also required for migration of T cells that is dependent on the integrin LFA-1. Invasion of TAM2D2 T cell hybridoma
Global Trade Item Number
| SKU | GTIN |
|---|---|
| Z2126-20UG | 04061838073266 |