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Merck
CN

50487-U

Ascentis® Express 90 Å Phenyl-Hexyl (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core Phenyl HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
phenylhexyl phase
Pore size:
90 Å pore size
Matrix:
Fused-Core particle platform, superficially porous particle
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Product Name

Ascentis® Express Phenyl-Hexyl, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Quality Level

agency

suitable for USP L11

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable

L × I.D.

25 cm × 4.6 mm

surface area

90 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

phenylhexyl phase

particle size

5 μm

pore size

90 Å pore size

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

General description

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

Application


  • A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers.: This study by Lhotská et al. explores the application of a fused-core column combined with on-line SPE-HPLC for rapid and automated analysis of ochratoxin A and citrinin in lager beers. The Ascentis® Express Phenyl-Hexyl column demonstrates high efficiency and selectivity, significantly improving the throughput and sensitivity of the analysis (Lhotská et al., 2016).

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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