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Supelco

Supelclean™ ENVI-Carb™ SPE Tube

bed wt. 250 mg, volume 3 mL, pk of 54

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material

polypropylene tube

Quality Level

100

product line

Supelclean™

composition

bed wt., 250 mg

packaging

pk of 54

technique(s)

solid phase extraction (SPE): suitable

surface area

100 m2/g

volume

3 mL

matrix active group

carbon phase

particle size

120-400 mesh

application(s)

food and beverages

separation technique

reversed phase

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General description

Solid phase extraction apparatus uses glass vials or tubes for sample collection and concentration. Some sorbents require large desorption volume than small SPE cartridges in such case SPE tubes were used.
Graphitized Non-Porous Carbon

Retention Mechanism: Reversed-phase
Sample Matrix Compatibility: Aqueous solutions (drinking, ground, waste water)

  • Surface area: 100m2/g; Particle Sz.:120/400 mesh
  • Extreme affinity for organic polar and non-polar compounds from both non-polar and polar matrices when used under reversed-phase conditions
  • Carbon surface comprised of hexagonal ring structures, interconnected and layered into graphitic sheets
  • Non-porous nature of the carbon phase allows for rapid processing, adsorption does not require analyte dispersion into solid phase pores
  • Independent investigators have found ENVI-Carb extremely useful for the rapid sample preparation of over 200 pesticides from various matrices including ground water, fruits and vegetable

Application

Supelclean™ ENVI-Carb™ SPE Tube was used in separating glycan moiety of glycosphingolipids from ceramide, salt and detergents. It was also performed to extract uridine diphosphate (UDP)-sugars from plant extract and reduce the sample complexity.

Legal Information

ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC
Supelclean is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Robert Behmüller et al.
Analytical and bioanalytical chemistry, 406(13), 3229-3237 (2014-03-19)
An analytical workflow was developed for the absolute quantification of uridine diphosphate (UDP)-sugars in plant material in order to compare their metabolism both in wild-type Arabidopsis thaliana and mutated plants (ugd2,3) possessing genetic alterations within the UDP-glucose dehydrogenase genes involved
Yao-Yun Fan et al.
The Journal of biological chemistry, 283(24), 16455-16468 (2008-04-17)
Mammalian glycan chain elongation is mostly based on extending the type 2 chain, Galbeta1-4GlcNAc, whereas the corresponding type 1 chain, Galbeta1-3GlcNAc, is not normally extended. In a broader context of developing high sensitivity mass spectrometry methodologies for glycomic identification of
Nigel J.K. Simpson
Solid-Phase Extraction: Principles, Techniques, and Applications, 445-445 (2010)

Articles

Solid Phase Extraction: Reversed-Phase Methodology

Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.

Related Content

Solid Phase Extraction (SPE)

Solid phase extraction (SPE) is a technique used for rapid, selective sample preparation and purification prior to chromatographic analysis (e.g. HPLC, GC, TLC). SPE is used to exchange sample matrices, concentrate analytes, and remove interferences and contaminants to improve data and protect the analytical column.

Chromatograms

application for SPE, application for HPLC

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