Protein purification

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facet applications:Protein purification

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Performing a Purification of IgG Antibodies with Protein A Mag Sepharose® Xtra or Protein G Mag Sepharose® Xtra

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Column Packing and Preparation

This page describes efﬁcient column packing and preparation for affinity chromatography of tagged proteins using GE Healthcare products.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins

This page describes efﬁcient column packing and preparation for affinity chromatography of GST-tagged proteins using GE Healthcare products.

Gravity-ﬂow Puriﬁcation using His GraviTrap™ and His GraviTrap™ Kit

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from GE Healthcare.

Cell disruption and membrane preparation

This page shows how to perform a cell disruption und prepare membranes with products from GE Healthcare.

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

Puriﬁcation of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using HisTrap™ Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from GE Healthcare.

Antibody Purification using Protein A, Protein G, or Protein L Agarose

Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum

Performing a Purification with Calmodulin Sepharose 4B

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from GE Healthcare.

General Instructions for Affinity Purification Using HiTrap® Columns

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Purification or Removal of Glycoproteins and Polysaccharides

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from GE Healthcare.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Puriﬁcation of Histidine-Tagged Proteins using TALON® Superﬂow

This page shows how to purify histidine-tagged proteins using TALON Superﬂow from GE Healthcare.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Refolding using ion exchange chromatography and analysis of refolding

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

Performing a Separation with Superdex

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Purification of Histidine-Tagged Proteins using HiTrap IMAC HP and HiTrap IMAC FF

This page shows how to purify histidine-tagged proteins using HiTrap IMAC HP and HiTrap IMAC FF from GE Healthcare.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Handling Inclusion Bodies in Recombinant Protein Expression

This page how to isolate proteins from inclusion bodies using GE Healthcare products.

SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

Performing a Separation or Removal of Albumin with HiTrap Blue HP and Blue Sepharose 6 Fast Flow

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

Performing an Isolation of Native Complexes

This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from GE Healthcare.

cOmplete™ His-Tag Purification Resin Protocol & Troubleshooting

Removal of Albumin Using Blue Sepharose® Chromatography Media

This page shows how to remove albumin from afﬁnity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from GE Healthcare.

Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance

Rapid Antibody Concentration with Amicon® Ultra Centrifugal Filters

Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.

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