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Large molecule HPLC
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2 Micron SEC Column with Dual Functionality for Use in UHPLC and HPLC Systems
These columns are designed to be operated with a simple and well established method (sodium phosphate mobile phase, pH 6.8) and are packed for use in both HPLC and UHPLC systems.
Ascentis® Express C18 U/HPLC Columns
Ascentis® Express C18 is the first choice when starting a new separation method.
Chromatography Profile Analysis of Phosphorothioate Oligonucleotides
The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular
Ascentis ® Express HILIC U/HPLC Columns
For separation of polar compounds including polar neutrals, polar acids, and polar and non-polar basic amines use our Ascentis® Express HILIC column.
Methods and Matrices for Mass Spectrometry of Glycans
Glycosylation is known to have profound influence on various physiochemical, cellular and biological functions of proteins. Alterations in this modification are known to affect the immune system and have been associated with various pathological states such as cancer, rheumatoid arthritis
Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard
Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.
Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
Oligonucleotide Quality Control by Analytical HPLC
In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.
Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the
HPLC Analysis of Methylphenobarbital (Mephobarbital) Enantiomers on Astec® CHIROBIOTIC® V
Separation of Mephobarbital, United States Pharmacopeia (USP) Reference Standard
Dyestuff Analyses on Historic Objects by LC-ESI-MS/MS Using a Superspher RP-18 HPLC Column
In this study, LC-ESI-MS/MS under reversed phase conditions using a Superspher RP-18 column is applied to analyze dyestuffs of a reddish historic textile from South America.
Columns and Analytical Standards for Protein SEC
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns
Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan
HPLC Analysis of Pain Relievers on Ascentis® Express C18
Separation of Acetaminophen, meets USP testing specifications, 98.0-102.0%, powder; Caffeine, meets USP testing specifications, anhydrous; Benzoic acid, meets USP testing specifications; Aspirin, meets USP testing specifications
Chromolith® HPLC and UHPLC columns
Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.
How to Maximize Sensitivity in LC-MS
Various measures and options for maximized LC-MS sensitivity and low limit of detection (LOD) are shown on this page. Each and every tip avoids contaminations causing signal suppression, adduct formation, elevated background noise and increased spectrum complexity.
HPLC Analysis of Z-Drugs on Ascentis® Express ES-Cyano 2.0 μm
An rapid method for the siµLtaneous determination of the Z-drugs or sleep aids: zopiclone, zolpidem, and zaleplon is presented here. The need for greater analytical capacity and throughput for the analysis of sleep aid medicines (Z-drugs) in forensic toxicology laboratories
UHPLC/MS Analysis of Spice Cannabinoids on Titan™ C18
Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provides a cannabis-type high.
Supelco Preparative HPLC products for Pharmaceutical Development and Production
Supelco Preparative HPLC products for Pharmaceutical Development and Production
Ascentis® Express C8 U/HPLC Columns
When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.
HPLC Columns for Antibody and Large Protein Analysis
BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.
Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis
Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith®
Ascentis® Express RP-Amide Columns for HPLC and UHPLC
Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.
How to Extend Liquid Chromatography Column Life: Regenerate or Use Guard Columns
What is causing aging of a column? Is the regeneration/washing of the column a reasonable approach to extend the lifetime of a column? What are the risks & challenges? Learn more in this article.
Ascentis® Express Biphenyl UHPLC and HPLC Columns
The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the
High Resolution and High Efficiency Separations of mAbs and ADCs Using Proteomix HIC Columns
Hydrophobic interaction chromatography, a protein separation technique, has been commonly used as an orthogonal method to SEC and ion exchange for the characterization of mAbs.
Ascentis® Express F5 (pentafluorophenyl) U/HPLC Columns
Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds
LC/MS Analysis of Illicit Bath Salts on Ascentis® Express HILIC, Fast Analysis
Separation of Butylone hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material; Ethylone hydrochloride, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material; Mephedrone hydrochloride solution, 1.0 mg/mL
Optimising the LC-MS Analysis of Biomolecules
Sigma-Aldrich.com presents an article concerning optimising the LC-MS analysis of biomolecules.
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