Merck
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TLC Analysis of Cholesteryl Esters on HPTLC Si 60 F254 with Concentrating Zone

TLC Analysis of Cholesteryl Esters on HPTLC Si 60 F254 with Concentrating Zone application for TLC

Materials

accessory

Product No.
Description
Pricing

Bottle-type sprayer

capacity 240 mL

Rectangular TLC developing tanks, complete

L × H × W 27.0 cm × 26.5 cm × 7.0 cm

Sigma TLC spray box

Spectroline® E-Series UV lamp

output8 W, AC/DC input 115 V AC

Rectangular TLC developing tanks, complete

L × H × W 7.5 cm × 15.5 cm × 8.0 cm

Hamilton® TLC syringes

1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),

coated tip

Spectroline® E-Series UV lamp

output6 W, AC/DC input 120 V AC

Hamilton® TLC syringes

1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),

coated tip

Hamilton® TLC syringes

1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),

coated tip

Rectangular TLC developing tanks, complete

L × H × W 17.5 cm × 16.0 cm × 8.2 cm

Spectroline® E-Series UV lamp

output6 W, AC/DC input 230 V AC

Spectroline® E-Series UV lamp

output4 W, AC/DC input 230 V AC

Spectroline® E-Series UV lamp

output8 W, AC/DC input 230 V AC

Latch-lid TLC developing chambers

For use with 10 cm × 10 cm plates

Hamilton® TLC syringes

1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),

coated tip

Latch-lid TLC developing chambers

For use with 20 cm × 20 cm plates

Spectroline® E-Series UV lamp

output4 W, AC/DC input 115 V AC

Kimble® microcapillary pipettes

Kimble® microcapillary pipettes

Kimble® microcapillary pipettes

capacity 20 μL, accuracy: ±0.5%

Reagent sprayer for TLC plates

volume 250 mL, Bottle-Type

CONDITIONS

plate

HPTLC pre-coated plate silica gel 60 F254 with concentrating zone (1.13728.0001, 1.13727.0001)

eluent

[A] toluene; [B] petroleum benzene 40-60 °C; (40:60, A:B)

migration distance

7 cm

chamber

normal chamber without chamber saturation

sample

5 μL

detector

in situ evaluation with TLC/HPTLC scanner (Camag) UV 366 nm. (spray reagent: MnCl2-sulfuric acid heated up to 110° C for 5 min)