CN
TLC Analysis of Sugars on Silica gel 60 (analyte set 2)
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
1.05641 HPTLC Silica gel 60, 20 cm x 10 cm (1.05641.0001)
eluent
2-propanol/chloroform/methanol/ammonia(25%)/water (45/45/20/4/6) for 7 cm followed by drying the plate; (then water/acetonitrile (15/85) for 7 cm followed by drying the plate (repeat three times with drying between each))
migration distance
7 cm each plate
chamber
normal chamber without chamber saturation
sample
2 μL
detector
Blei(IV)-acetat-Dichlorfluorescein-Reagent prepare a mixture of 3,75ml Blei(IV)-acetat saturated in glacial acetic acid and 3,75ml ( 0,22g Dichlorfluorescein in 100ml ethanol) (and dilute to 150ml with toluene, spray 20s and heat 30min at 100°C, Detection UV @ 366nm)