The appearance of uric acid is measured at 293nm by spectrophotometry.
One unit causes the formation of one micromole of uric acid per minute under the conditions described below.
Reagents
At the same time, measure the blank rate (ΔOD blank) by using the same method as the test except that the enzyme diluent (E) is added instead of the enzyme solution.
* Dissolve the enzyme preparation in ice-cold enzyme diluent (E), dilute to 0.1-0.2U/mL with the same buffer and store on ice.
Activity can be calculated by using the following formula:
Volume activity (U/mL) = |
|
|
= |
Δ OD/min x 2.0 x df |
Weight activity (U/mg)=(U/mL)×1/C
This procedure is for informational purposes. For a current copy of our quality control procedure, please contact our Technical Service Department.
To continue reading please sign in or create an account.
Don't Have An Account?