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安全信息

126575

Sigma-Aldrich

白蛋白,牛血清,组分V,不含脂肪酸

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别名:
白蛋白,牛血清,组分V,不含脂肪酸
CAS号:

检测方案

≥95% (cellulose acetate electrophoresis)

形式

powder

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

杂质

≤0.02% fatty acids
≤3% ash
≤5% moisture

颜色

white to light yellow
light yellow to light brown

运输

ambient

储存温度

2-8°C

一般描述

牛血清白蛋白(BSA、组分V、不含脂肪酸)适用于免疫诊断分析。它设计用于血清学检测、放射免疫测定和激素反应研究。它也适用于结合和运输研究。BSA采用冷乙醇提取法(Cohn法)制备。pH值:6.8-7.4。
牛血清白蛋白(BSA)是一种非糖基化球状α-螺旋蛋白,属于血清白蛋白家族。它由三个结构域组成,每个结构域有两个子结构域,并且具有17个二硫键。

应用

牛血清白蛋白(组分V、不含脂肪酸)已用作:
  • 一种组分以用于各种细胞免疫电子显微镜(IEM)的阻断缓冲液
  • 线粒体隔离缓冲液(MIB)和线粒体测定液(MAS),以捕获脂肪组织均质化过程中释放的游离脂肪酸
  • 一种阻断缓冲液,以对食管上皮(EE)进行免疫荧光全旦染色

生化/生理作用

牛血清白蛋白(BSA)是细胞培养基中最重要的成分,有助于胚胎干细胞(hESC)的分化。它有助于药物、激素和脂肪酸的运输。BSA也被用作酶联免疫吸附试验(ELISA)的阻断剂。

警告

毒性:标准处理(A)

制备说明

可能需要长达24小时才能完全溶解。

重悬

在复溶后存放于冰箱(4°C)。可能添加0.1% NaN₃作为防腐剂。储备溶液在4°C下可稳定保存3个月。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

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T Akao et al.
Journal of biochemistry, 99(5), 1425-1431 (1986-05-01)
Ruminococcus sp. PO1-3 obtained from human intestinal flora is able to reduce dehydrocholate as well as 3-ketoglycyrrhetinate. From this bacterium dehydrocholate- and 3-ketoglycyrrhetinate-reducing activities were purified one thousand-fold together with 3-ketocholanate-reducing and 3-beta-hydroxyglycyrrhetinate (glycyrrhetic acid) oxidizing activities by means of
Xiao Qun Wang et al.
Lipids, 46(6), 479-486 (2011-02-22)
Excessive formation of advanced glycation end products (AGE) and lipid accumulation in macrophages play a pivotal role in the progression of atherosclerosis in diabetes mellitus. This study aimed to determine the molecular link between AGE-induced fatty acid binding protein 4
Saeko Akiyama et al.
Stem cell research & therapy, 13(1), 104-104 (2022-03-14)
The liver plays an important role in various metabolic processes, including protein synthesis, lipid and drug metabolisms and detoxifications. Primary culture of hepatocytes is used for the understanding of liver physiology as well as for the drug development. Hepatocytes are
Erica L Scheller et al.
Nature communications, 6, 7808-7808 (2015-08-08)
Marrow adipose tissue (MAT) accumulates in diverse clinical conditions but remains poorly understood. Here we show region-specific variation in MAT adipocyte development, regulation, size, lipid composition, gene expression and genetic determinants. Early MAT formation in mice is conserved, whereas later
Alexandros Tzatsos et al.
The Journal of biological chemistry, 282(25), 18069-18082 (2007-04-27)
Energy depletion activates AMP-activated protein kinase (AMPK) and inhibits cell growth via TSC2-dependent suppression of mTORC1 signaling. Long term energy depletion also induces apoptosis by mechanisms that are not well understood to date. Here we show that AMPK, activated by

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