PTP Assay Kit 1

PTP Assay Kit 1 can be used to detect PTP-1B activity by dephosphorylation of the phosphopeptide (RRLIEDAEpYAARG) using malachite green detection, or by hydrolysis of pNPP.

Used to detect/quantify: PTP

Kit capacity: 100 assays

routinely evaluated by malachite green and pNPP hydrolysis assays to detect PTP-1B (Catalog # 14-109 or Catalog # 14-358) activity by dephosphorylation of the phosphopeptide

Tyrosine Phosphopeptide (RRLIEDAEpYAARG] (Cat.# 12-217)

Malachite Green Solution A (Cat.# 20-105)

Malachite Green Additive (Cat.# 20-104)

Phosphate Standard (Cat.# 20-103)

pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)

pNPP Tyr Assay Buffer (Cat.# 20-180)

96-well microtiter plate

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