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Merck
CN

CC130

Human Vitronectin

recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM®

别名:

S-protein, Serum-spreading factor, V75

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关于此项目

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
Escherichia coli, human
Form:
liquid
Technique(s):
cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable
Concentration:
0.5 mg/mL
Assay:
≥92% (SDS-PAGE)
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产品名称

PluriSTEM-XF® 重组玻连蛋白, Human pluripotent stem cell culture.

Quality Level

biological source

Escherichia coli, human

assay

≥92% (SDS-PAGE)

form

liquid

manufacturer/tradename

PluriSTEM®

concentration

0.5 mg/mL

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable

input

sample type: human embryonic stem cell(s)

UniProt accession no.

shipped in

dry ice

storage temp.

-10 to -25°C

General description

PluriSTEM 重组玻连蛋白是matrigel的有效无异种替代品,已被验证可以使用PluriSTEM人ES/iPS培养基(Millipore目录编号SCM130,SCM132),E8和NutriStem人ES细胞生长培养基成功培养人ES和iPS细胞。

PluriSTEM重组玻连蛋白是人玻连蛋白(基因20-398aa片段)的截短形式,其通过密码子优化构建,在大肠埃希菌中表达并纯化为包含体。使用独特的“温度变化包涵体重折叠”技术将最终产物重折叠并进行色谱纯化。最终产品被认为是无异种的。

Application

支持人es/ips细胞的无饲养层和无血清生长,同时维持无异种培养条件。
研究类别
干细胞研究

细胞结构

ECM & 粘连

Physical form

在含有专有处方NaCl、KCl、EDTA、精氨酸、DTT和甘油的20 mM pH值8.0 Tris-HCl缓冲液中对产品进行无菌过滤。

Preparation Note

储存于-20°C,直到小瓶上的有效期为止。 解冻后,产品在2-8°C下可稳定储存两周。 解冻后的玻连蛋白可分装于-20°C保存。 应避免其他冻融循环。

Analysis Note

在每孔1 mL人ESC培养基(6孔板)中使用5-10 μg重组玻连蛋白培养的人ES细胞(H1)上测试每个批次。

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany


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flash_point_f

Not applicable

flash_point_c

Not applicable

存储类别

12 - Non Combustible Liquids



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实验方案

产生人诱导多能干细胞(iPSC)的干细胞重编程实验方案,包括基于病毒和非病毒RNA的方法。

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

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商品

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.





全球贸易项目编号

货号GTIN
CC13004053252938511