species reactivity
vertebrates
Quality Level
technique(s)
cell based assay: suitable
detection method
colorimetric, fluorometric
Application
The Millipore QCM 3 μm Endothelial Cell Migration Assay – Fibronectin, Fluorometric is ideal for the study of endothelial cell migration in response to an angiogenic stimulus. The quantitative nature of this assay is especially useful for large scale screening of pharmacologic agents. BSA-coated control chambers provide an appropriate migration control. The 3 μm pore size in this assay is optimal for endothelial cells such as HUVEC, but not sufficient for fibroblast migration. The Millipore QCM 3 μm Endothelial Cell Migration Assay – Fibronectin, Fluorometric assay is intended for research use only; not for diagnostic applications.
Each kit provides sufficient materials for the evaluation of 12 samples.
Each kit provides sufficient materials for the evaluation of 12 samples.
Packaging
Sufficient for 12 assays
Preparation Note
- The ECM201-1 kit components should be stored at 2° to 8°C up until the expiration date provided on the labels. DO NOT FREEZE.• The accompanying 100ml bottle of Accutase® also included in this kit should be stored at -20°C.
Other Notes
1. Fibronectin Test Plate: One 24-well culture plate, containing 12 human FN-coated Boyden chambers, sufficient for the evaluation of 12 test samples. 2. BSA Control Plate: One 24-well culture plate, containing 12 BSA-coated Boyden chambers, sufficient for the evaluation of 12 controls. 3. 4X Cell Lysis Buffer - One 16 mL bottle 4. CyQuant GR™ Dye: One vial 75 μL 5. 24-Well Cell Culture Tray 6. 96-Well Stain Quantitation Plate 7. Forceps: 1 pair 8. Accutase® (cell detachment solution): One 100 mL bottle
Legal Information
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
GR is a trademark of Sigma-Aldrich Co. LLC
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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