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Merck
CN

MABT493

Anti-IL-34, clone 1D12 Antibody

ascites fluid, clone 1D12, from mouse

别名:

Interleukin-34, IL-34

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1D12, monoclonal
Application:
FACS, IF
Citations:
2
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1D12, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable, immunofluorescence: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IL34(146433)

General description

27 kDa calculated
Intereukin 34, also known as IL-34 or IL34 is a cytokine that promotes the differentiation and viability of moncytes and macrophages. IL-34 stimulate the phosphorylation of MAPL1/ERK2/ERK2 and MAPK3/ERK1. IL-34 is a ligand for colony-stimulating factor CSF1R. IL-34 is predominantly expressed in spleen, and also detected in a range of other tissues including heart, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

Immunogen

Purified recombinant fragment of human IL-34 expressed in E. Coli

Application

Anti-IL-34, clone 1D12 Antibody is an antibody against IL-34 for use in flow cytometry & immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected IL-34 in U251 cells.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected IL-34 in K562 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable



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Muhammad Baghdadi et al.
Inflammation and regeneration, 38, 12-12 (2018-07-14)
Cysts of the salivary glands are common lesions that occur in the context of various etiologies. Although the diagnostic importance of cysts in salivary gland diseases has been well studied, molecular mechanisms that control the related pathological process remain largely
Nanumi Han et al.
STAR protocols, 2(2), 100460-100460 (2021-04-27)
Previously, we identified a therapy-resistant role of IL-34 in an immune checkpoint blockade in murine models. To investigate whether a similar mechanism is applicable in human tumors as well, we used this protocol for the selection of IL-34-neutralizing antibody and



全球贸易项目编号

货号GTIN
MABT49304053252955471