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OP43

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

Name: Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)
Brand: MM

liquid, clone DO-1, Calbiochem^®

别名:

Anti-p53 Antibody, Mouse Anti-p53, p53 Detection Antibody

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关于此项目

NACRES:

NA.43

UNSPSC Code:

12352203

Clone:

DO-1, monoclonal

Species reactivity:

feline, human

Application:

—

Citations:

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属性

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

DO-1, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

feline, human

should not react with

mouse, rat

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

dilution

(Frozen sections (1 µg/mL)
Immunoblotting (0.1-1 µg/mL)
Immunocytochemistry (1-2.5 µg/mL)
Immunoprecipitation (1 µg/mL)
Paraffin sections (1 µg/mL, pepsin, heat or pressure cooker pre-treatment required))

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

说明

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X63Ag8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.

Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.

This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).

Immunogen

Epitope: Within amino acids 21-25 of human p53

Human

wild type, recombinant, human p53

Application

Frozen sections (1 g/ml; see application references)
Immunoblotting (0.1-1 g/ml; see application references)
Immunocytochemistry (1-2.5 g/ml; see application references)
Immunoprecipitation (1 g/ml or use Cat. No. OP43A; see application references)
Paraffin sections (1 g/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative Control
SK-OV-3 cells or normal skin tissue

Positive Control
A431 cells or breast carcinoma tissue

Other Notes

El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Vojtesek, B., et al. 1992. J. Immunol. Meth.151, 237.
Kastan, M.B., et al. 1991 Cancer Res.51, 6304.

Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 μl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with ³⁵S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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存储类别

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c