Merck
CN

12106C

Sigma-Aldrich

胎牛血清

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

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别名:
FBS, FCS, 血清
MDL编号:
NACRES:
NA.71

生物来源

bovine fetus

质量水平

无菌性

sterile-filtered

产品线

FBS Premier

组成

hemoglobin, ≤20 mg/dL

来源

USA origin

技术

cell culture | mammalian: suitable

杂质

≤10 EU/mL endotoxin

运输

dry ice

储存温度

−20°C

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应用

胎牛血清(FBS)可为培养细胞提供了多种非明确的生长促进和存活增强因子。热灭活 FBS 可用于代替常规 FBS,其中补体系统的灭活有利于细胞生长或功能。热灭活 FBS 用于培养利什曼原虫

制备说明

在56°C加热30分钟制备。
在56°C加热30分钟制备。将血清加热至56°C并保持30分钟可灭活补体系统以进行免疫测定。热激活还会使培养物中其他不明细胞生长抑制剂失活。

分析说明

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

新产品

分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Localized cutaneous leishmaniasis (LCL) in India is due mostly to Leishmania tropica. It is mainly endemic in the deserts of Rajasthan. Recently, Himachal Pradesh has been identified as a new endemic focus for the disease. In the last few years
Barbara Belletti et al.
Molecular biology of the cell, 19(5), 2003-2013 (2008-02-29)
The balanced activity of microtubule-stabilizing and -destabilizing proteins determines the extent of microtubule dynamics, which is implicated in many cellular processes, including adhesion, migration, and morphology. Among the destabilizing proteins, stathmin is overexpressed in different human malignancies and has been
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Neurotrophic factors control neural cell differentiation and assembly of neural circuits. We previously showed that organophosphate pesticides differentially regulate members of the fibroblast growth factor (fgf) gene family. We administered chlorpyrifos and diazinon to neonatal rats on postnatal days 1-4

实验方案

WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.

WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

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