Merck
CN
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安全信息

14365C

SAFC

EX-CELL® CD CHO Fusion

Animal-component free, without L-glutamine, without hypoxanthine, without thymidine, liquid, sterile-filtered, suitable for cell culture

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别名:
EX-CELL® CD CHO Fusion
NACRES:
NA.75

质量水平

500

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

组分

L-glutamine: no

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

EX-CELL® CD CHO Fusion is a chemically defined, animal-component free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells. The absence of any large macro molecules allows for isolation and purification of secreted proteins from the cells. This medium is supplied without L-glutamine to aid in media stability, to avoid L-glutamine degradation that causes ammonia build-up and to provide an appropriate medium for the culture of CHO cells using glutamine synthetase selection (example: MilliporeSigma’s CHOZN GS knockout cell line). This medium does not contain hypoxanthine or thymidine to allow for its use with dihydrofolate reductase(DHFR-) gene amplification systems (example: MilliporeSigma’s CHOZN DHFR knockout cell line).

应用

EX-CELL ® CD CHO FUSION 是一种化学限定、无动物成分的培养基，为中国仓鼠卵巢 (CHO) 细胞的长期生长而开发。培养基中无大分子，可实现细胞分泌蛋白的分离和纯化。

本品基于以下目的不含 L-谷氨酰胺：有助于培养基的稳定性，避免因 L-谷氨酰胺降解引起氨积累，以及为采用谷氨酰胺合成酶或 GS System™ 培养 CHO 细胞时提供适用的培养基。该培养基不含次黄嘌呤或胸腺嘧啶核苷，可与二氢叶酸还原酶 (DHFR) 基因扩增系统一同使用。

数量

Formulated to contain 20.9 grams of powder per liter of medium.

法律信息

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Qiong Wang et al.
Biotechnology and bioengineering, 116(9), 2303-2315 (2019-05-08)
With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing. Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the
Noriko Yamano-Adachi et al.
Scientific reports, 10(1), 17612-17612 (2020-10-21)
Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins.
Weiyi Liu et al.
Biotechnology progress, 37(1), e3061-e3061 (2020-08-05)
Antibody-dependent cellular cytotoxicity (ADCC) is the primary mechanism of actions for several marketed therapeutic antibodies (mAbs) and for many more in clinical trials. The ADCC efficacy is highly dependent on the ability of therapeutic mAbs to recruit effector cells such

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Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines at sigmaaldrich.com

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Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines at sigmaaldrich.com

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