Merck
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文件

安全信息

A7089

Sigma-Aldrich

ACCUMAX™ 溶液

sterile-filtered, suitable for cell culture

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别名:
Cell detachment solution
MDL编号:
MFCD01632904
NACRES:
NA.75

无菌性

sterile-filtered

质量水平

200

形式

solution

浓度

1 ×

技术

cell culture | mammalian: suitable
single cell analysis: suitable

pH值（酸碱度）

6.8-7.8

运输

dry ice

储存温度

−20°C

相关类别

应用

TM = [“Accumax”]溶液已用于
  • 从上皮细胞的细胞外基质(ECM)解离单壁碳纳米管
  • 在传代前解离视网膜色素层细胞
  • 从胰腺上解离胰岛细胞

Accumax已经证明能够在杂交瘤、CHO、BHK、293、COS和Sf9细胞的悬浮培养物中对成簇细胞进行有效解离。 该溶液在解离细胞计数、病毒转染测定、细胞分选、流式细胞术和生物反应器放大方面具有极佳的表现。 它还被用于从聚集的细胞培养物中获得单细胞悬浮液，以实现精确的细胞计数、细胞与原发组织的分离、以及细胞与标准组织培养塑料器皿和粘附涂层塑料器皿的常规分离。

生化/生理作用

Accumax是一种细胞分离溶液，由蛋白水解酶和胶原溶解酶组成。 每瓶含有100 mL溶于杜氏′PBS的1x Accumax酶。Accumax中没有发现哺乳动物或细菌衍生产物。

特点和优势

用于体外 细胞应用的即用型无菌液体

注意

Accumax在-20°C下储存可保持稳定。解冻后，产品可在4°C下储存长达2个月。

公式变量

在杜氏′PBS（0.2g/L KCl、0.2g/L KH2PO4、8g/L NaCl和1.15g/L Na2HPO4）中进行制备。 有关产品使用信息，请参阅网站上的产品数据表。

制备说明

本产品以无菌即用型冷冻液的形式提供。 Accumax应在使用前在37°C解冻，并以等体积加入至集聚细胞样品中。 在37°C下孵育5-10分钟，然后进行正常计数细胞。

法律信息

Accumax is a trademark of Innovative Cell Technologies, Inc.

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?

    Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells.  The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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